نظام يوم العلم العراقي 2019جائزة البحث العلمي المتميز
طبع بتاريخ 2020/04/09 بتوقيت 12:30:00pm
بيانات المتقدم للجائزة
اسراء محمدصفي عبدعلي
بيانات البحث المقدم للجائزة
Chitosan extracted from Aspergillus flavus shows synergistic effect,eases quorum sensing mediated virulence factors and biofilm againstnosocomial pathogen Pseudomonas aeruginosa link
International Journal of Biological Macromolecules link
A number of bacterial species produces chitosanases which has variety of applications because of its high biodegradability, non-toxicity and antimicrobial assets. In the present study chitosanase is puriﬁed from new bacterial species Bacillus licheniformis from spoiled vegetable. This novel strain of Bacillus lichen- iformis isolated from spoilt cucumber and pepper samples has the ability to produce the chitosanase enzyme when grown on chitosan substrate. Study also examined its antibioﬁlm properties against diverse bacterial species with bioﬁlm forming ability. The puriﬁed chitosanase inhibited the bioﬁlm formation ability for all Gram-negative and Gram-positive bioﬁlm-forming bacteria [bioﬁlm producers] tested in this study in congo red agar and microtiter plate's methods. Highly antibioﬁlm activity of chitosanase was recorded against Pseudomonas aeruginosa followed by Klebsiella pneumoniae with reduction of bioﬁlm formation upto 22 and 29%, respectively compared with  % of control. Bioﬁlm formation has multiple role including ability to enhance resistance and self-protection from external stress. This chitosanase has promising beneﬁt as antibioﬁlm agent against bioﬁlm forming pathogenic bacteria and has promising application as alternative antibioﬁlm agents to combat the growing number of multidrug resistant pathogen-associated infections, especially in situation where bioﬁlms are involved.