The current project focuses on the development and fabricated of micro-device using three different designed methods of filtration and separating for blood and cancer cells separation and filtration or for any other chemical substances particles separation.
The response surface methodology (RSM), the full factorial design (FFD) and the expert system 11.0 software program were selected to design the experimental work improved and verified the experimental results.
The experimental results showed that the blood and cancer cells filtration and separating quantities are increasing with increasing the air pressure level and the microfluidic pore size. The best results for the highest quantity of blood separated and free of tumor and cancer cells were obtained at 150 mbar air pressure and the use of horizontal separation system, which is higher by 282% compared with the use of the vertical separation system.
The highest quantity of blood white cell (BWC) separated were obtained at 150 mbar air pressure and the use of horizontal separation system, reached 17.14 mL., which is higher by 32% than when use of the microfluidic blood white cell (BWC) separation system and by 343 % compared with the use of the vertical separation system.
The largest quantity of blood red cell (BRC) separated were obtained at 150 mbar air pressure and the use of horizontal separation system, reached 5.71 mL., which is higher by 90 % compared with the use of the vertical separation system.The highest quantity of blood cell filtration from the virus cells separated were obtained at 100 mbar air pressure and the use of vertical separation system,
reached 2.50 mL., which is higher by 9 times compared with the use of the horizontal separation system.