This study included the diagnosis of Proteus mirabilis isolated from patients attended
different hospitals, laboratories and privet laboratories in the Hilla city/Iraq (Hilla
Teaching hospital, Childhood and Gynecology Hospital).
Fourteen isolates of P. mirabilis were idetifiction based on phenotypic, microscopic
and biochemical tests.
Some bacterial virulence factors related to Urinary tract infection have been
investigated. The results showed of isolates to the production of hemolycin , urease,
and formation of swarming.
The sensitivity test for all isolates of P. mirabilis were performed against six
antibiotics commonly used to treat P. mirabilis, including Amoxicillin, cefotaxime,
Amikacin, ceftazadim, Imipenem, and Merobenem. All isolates showed resistance
against Amoxicillin and Cefotaxime were 100%, while the other antibiotics Amikacin,
Ceftizidime, Meropenem, and Imipenem were 64.28%, 50%, 28.57% and 14.28%,
In order to confirm the results of the identification based on the biochemical tests, the
results were confirmed by molecular identification using ureR gene of P. mirabilis.
The bacterial DNA was extracted from 14 isolates and using the QIAGEN kit.
Out of 14 P. mirabilis isolates 7 isolates were positive for ureR; the other 7 isolates
were negative for this gene. This identification may be more accurate than the
phenotypic characterization which entails biochemical features.