The study clarifies the effect of combination (Chitosan nanoparticles + Methotrexate drug) on breast cancer cell line (MCF-7 Cell) and Inhibiting the growth of antimicrobial resistance (AMR) bacteria (S.aureus) which poses a future threat to the lives of patients.
The study included the manufacture and characterization of Chitosan nanoparticles to detect the synthesis of chitosan nanoparticles
The study aims to investigate the effects each of chitosan nanoparticles, Methotrexate drug (MTX), and the combination (chitosan NPs + MTX) on breast cancer cell MCF-7 and to Inhibit the growth of antimicrobial resistance (AMR) bacteria (S.aureus) as an alternative or adjunct to the work of antibiotics.
The results of this study showed an activity of combination (Chitosan NPs + Methotrexate) on MCF-7 cell cultures. It showed acceptable results for less cytotoxicity and its effect to inhibit high growth at low concentrations (8μg/ml) and is non-toxic to cells even at high concentrations.
Also, showed The combination (Chitosan NPs + Methotrexate) had a high synergistic effect with different concentrations (200, 100, 50 μg/ml) that were observed in Gram-positive bacteria at (26mm) in S.aureus with a concentration (200 μg/ml).
The study suggested that a Combination (Chitosan NPs + Methotrexate) is a good option for the most common causes leading to Methotrexate intoxication for breast cancer patients are inappropriate dosage, and concomitant use of drugs, such as antibiotics. For this reason, the Methotrexate drug was combined with chitosan nanoparticles to reduce the toxicity of the Methotrexate drug, increased efficiency, and also worked it on inhibiting microbes when infected.